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  • Title: Platelet-activating factor (PAF) induced cytokine production and otitis media with effusion (OME) in the rat middle ear.
    Author: Ogura M, Furukawa M, Tada N, Ikeda H, Yamashita T.
    Journal: Auris Nasus Larynx; 2008 Sep; 35(3):338-43. PubMed ID: 17997066.
    Abstract:
    OBJECTIVES: We examined the effect of platelet-activating factor (PAF) on IL-8 production in cultured rat middle ear epithelial cells (RMECs), and the concentrations of cytokine, PAF, and PAF-acetylhydrolase (PAF-AH) were also examined in the PAF-induced experimental OME (otitis media with effusion) of rats. METHODS: Using an enzyme-linked immunospecific assay, we measured the levels of cytokines in the cultured RMECs and the PAF-induced OME of rats. The PAF was quantitated by the platelet-aggregating activity and the PAF-AH was measured by radioimmunoassay. RESULTS: Both PAF and C-PAF, which is a stable analogue of PAF, significantly induced production of IL-8 in the RMECs in a dose-dependent manner. The PAF-induced IL-8 production was abolished by co-incubation with WEB2170, a specific PAF receptor antagonist. The concentrations of the cytokines and PAF in the PAF-induced OME of rats were higher on day 1 and the PAF and cytokine levels seemed to correspond well with the persistence of OME. CONCLUSION: PAF may stimulate the local production of cytokines and may induce OME in the middle ear.
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