These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Matrix metalloproteinases inhibitor doxycycline inhibits TGF-beta 1-induced RPE cell migration].
    Author: Zeng AP, Zeng SQ, Li PC, Cheng Y.
    Journal: Zhonghua Yan Ke Za Zhi; 2007 Aug; 43(8):734-8. PubMed ID: 18001573.
    Abstract:
    OBJECTIVE: To investigate the effects of matrix metalloproteinases (MMP) inhibitor (Doxycycline) on retinal pigment epithelial (RPE) cell migration induced by transforming growth factor beta 1 (TGF-beta 1). METHODS: The third to sixth passage human RPE cells cultures were treated with TGF-beta 1 at different concentrations (0.01, 0.10, 1.00, 10.00 microg/L), and the conditioned media were collected after 36 h of TGF-beta 1 exposure. Gelatinase activities in conditioned media were analyzed by zymography. Migration assay was performed in a Boyden chamber with addition of different concentrations of Doxycycline. The number of migrated cells was counted under light microscopy. RESULTS: RPE cells migration were stimulated by TGF-beta 1 significantly, the number of migrated RPE cells increased about 27% compared with the control (P < 0.01). In the presence of Doxycycline, the RPE cells migration induced by TGF-beta 1 was inhibited and the number of migrated cells was reduced to 50% - 70% compared with control (P < 0.01). TGF-beta 1 also stimulated the secretion of MMP-2 in dose-dependent manner. CONCLUSIONS: This study indicates that Doxycycline can inhibit migration of RPE cells stimulated by TGF-beta 1. RPE migration induced by TGF-beta 1 may act partially through the stimulation of MMP production.
    [Abstract] [Full Text] [Related] [New Search]