These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Possible role of hematopoietic CD44/chondroitin sulfate interaction in extravasation of peripheral blood CD16(-) natural killer cells into human endometrium.
    Author: Yasuo T, Kitaya K, Yamaguchi T, Fushiki S, Honjo H.
    Journal: J Reprod Immunol; 2008 Jun; 78(1):1-10. PubMed ID: 18006074.
    Abstract:
    Unique CD16(-) natural killer (NK) cells appear in the human cycling endometrium. Although their origin remains undetermined, one possible explanation is extravasation of circulating peripheral blood CD16(-) NK cells. Hematopoietic CD44 (CD44H) is an adhesion molecule expressed on leukocytes and plays a role in the initial step of leukocyte extravasation (leukocyte tethering/rolling). Recent studies have shown that CD44H binds to chondroitin sulfate (CS). To test the hypothesis that peripheral blood CD16(-) NK cells extravasate using the CD44H/CS interaction, we have compared the binding capacity of CD44H to immobilized CS among peripheral blood lymphocyte subsets, as well as determined the menstrual cycle-dependent expression of CD44H. Additionally, we have investigated the expression of the CS proteoglycan serglycin in human endometrial endothelial cells. CD44H expression on peripheral blood CD16(-) NK cells was higher compared with other lymphocyte subsets throughout the menstrual cycle. Peripheral blood CD16(-) NK cells bound preferentially to immobilized CS-A and CS-C compared with other lymphocyte subsets. The binding was significantly reduced by function-blocking anti-CD44H antibody. Serglycin expression in the human endometrial endothelial cells was greater in the secretory phase than in the proliferative phase. Progesterone (10(-7) M to 10(-8) M) significantly increased serglycin core protein expression in cultured human uterine microvascular endothelial cells, whereas 17beta-estradiol had no effect. These results indicate that the interaction between CD44H on peripheral blood CD16(-) NK cells and CS on endometrial endothelial cells may play a role in extravasation of these NK cells into human endometrium. Serglycin may be a potential CS proteoglycan involved in this phenomenon.
    [Abstract] [Full Text] [Related] [New Search]