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  • Title: [Determination of chyodeoxycholic acid in Bile Arisaema by HPLC-ELSD].
    Author: He Y, Zhang YX, Zhao HD, Zhang QW.
    Journal: Zhongguo Zhong Yao Za Zhi; 2007 Aug; 32(16):1634-6. PubMed ID: 18027654.
    Abstract:
    OBJECTIVE: To develop an HPLC method for the determination of hyodeoxycholic acid in Bile Arisaema. METHOD: The methanol extracts were separated on a Diamonsil ODS column eluted at 35 degrees C with a mixture of methanol-water-glacial acetic acid (75:25:0.01) at flow-rate 1.0 mL x min(-1). The drift-tube temperature of ELSD was 85 degrees C, and the flow rate of air was 3.0 L x min(-1). RESULT: Hyodeoxycholic acid in methanol extract was well separated, the relationship of logarithms of injection amount and peak area was linear (r = 0.9998) over the range of 2.4-24 microg. The average recovery was 97.1% (RSD 2.9%, n=5). CONCLUSION: The method could be used for quality control of Bile Arisaema.
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