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  • Title: Sensing of nucleic acid sequences using unmodified nucleic acid as a probe.
    Author: Narita A, Sando S, Aoyama Y.
    Journal: Nucleic Acids Symp Ser (Oxf); 2007; (51):281-2. PubMed ID: 18029696.
    Abstract:
    We present a strategy to generate a light-up fluorophore-aptamer pair. The strategy was based on a modification of a conventional DNA-staining dye to suppress its affinity to the original targets, and subsequent re-selection of aptamers that would bind to the modified dye. Along this line, we prepared an environmental polarity-sensitive Hoechst derivative with low affinity to the usual AT-rich dsDNA targets. DNA aptamers, in vitro selected from a random pool, worked as a trigger to enhance the fluorescence of an otherwise nonfluorescent Hoechst derivative.
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