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  • Title: Selection and characterization of DNA aptamers against VEGF165 with aptamer blotting method and its application.
    Author: Ikebukuro K, Hasegawa H, Sode K.
    Journal: Nucleic Acids Symp Ser (Oxf); 2007; (51):399-400. PubMed ID: 18029755.
    Abstract:
    We have isolated two DNA aptamers against a tumor marker protein, human vascular endothelial growth factor (VEGF165). To select aptamers that have high specificity for VEGF165, we used another protein as a competitor in the SELEX process. In addition, we evaluated the screened library with aptamer blotting. Isolated aptamers bound to VEGF165 with Kd in a few hundred-nanomolar ranges, and did not bind to a competitor. This selection method allowed us to select the specific aptamers against a target protein efficiently. Furthermore, isolated aptamers did not bind to VEGF121. These specific DNA aptamers against VEGF165 would be a useful sensing element for cancer diagnosis.
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