These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Construction of a gene coding for the precursor of human tumor necrosis factor].
    Author: Korobko VG, Selezneva II, Shingarova LN, Filippov SA, Dobrynin VN.
    Journal: Bioorg Khim; 1991 Oct; 17(10):1365-8. PubMed ID: 1804120.
    Abstract:
    The cDNA sequence for human tumor necrosis factor (hTNF) was reconstructed in vitro from genomic sequence. Using the oligonucleotide directed mutagenesis, a site for restriction endonuclease ClaI was introduced into the end of the first exon. The nucleotide sequence representing the second and third exons flanked with restriction sites ClaI and XhoI was obtained by means of chemical enzymatic synthesis. Assembly of the total gene coding for precursor of hTNF was accomplished in pTNF33 plasmid containing semisynthetic gene for mature hTNF with appropriate restriction sites.
    [Abstract] [Full Text] [Related] [New Search]