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  • Title: [Determination of macrozamin in Rhizoma Heterosmilacis Japonicae by RP-HPLC].
    Author: Qiao L, Chen XH, Bi KS.
    Journal: Zhongguo Zhong Yao Za Zhi; 2007 Sep; 32(18):1900-2. PubMed ID: 18051902.
    Abstract:
    OBJECTIVE: To develop a RP-HPLC method for the determination of the content of macrozamin in Rhizoma Heterosmilacis Japonicae. METHOD: A Century C18 AQ column (4.6 mm x 250 mm, 5 microm) was used with the mobile phase consisted of water (4:96). The flow rate was 1.0 mL x min(-1). The detection wavelength was set at 215 nm, and the column temperature was 35 degrees C. RESULT: The calibration curve was linear (r = 0.999 8) in the range of 19.12 - 382.4 microg x mL(-1) for macrozamin, the average recovery of the method was 99.5%, with RSD 2.1% (n = 9). CONCLUSION: This method can be used for the quality study of Rhizoma Heterosmilacis Japonicae.
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