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  • Title: Quantitative comparison between microfluidic and microtiter plate formats for cell-based assays.
    Author: Yin H, Pattrick N, Zhang X, Klauke N, Cordingley HC, Haswell SJ, Cooper JM.
    Journal: Anal Chem; 2008 Jan 01; 80(1):179-85. PubMed ID: 18052343.
    Abstract:
    In this paper, we compare a quantitative cell-based assay measuring the intracellular Ca2+ response to the agonist uridine 5'-triphosphate in Chinese hamster ovary cells, in both microfluidic and microtiter formats. The study demonstrates that, under appropriate hydrodynamic conditions, there is an excellent agreement between traditional well-plate assays and those obtained on-chip for both suspended immobilized cells and cultured adherent cells. We also demonstrate that the on-chip assay, using adherent cells, provides the possibility of faster screening protocols with the potential for resolving subcellular information about local Ca2+ flux.
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