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  • Title: [Production of monoclonal antibodies to Cymbidium mosaic virus and application in orchids virus detection].
    Author: Meng CM, Wu JX, Xie L, Zheng JK, Hong J.
    Journal: Wei Sheng Wu Xue Bao; 2007 Oct; 47(5):928-31. PubMed ID: 18062276.
    Abstract:
    Three hybridoma cell lines, 2C6, 5B7 and 12G9, secreting monoclonal antibodies (McAbs) against Cymbidium mosaic virus (CymMV) were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/ C immunized by the CymMV particles. The three McAbs could specifically react with CymMV. The titres of ascitic fluids of two McAbs are up to 10(-6) in I-ELISA. Isotypes and subclasses of the the three McAbs belong to IgG1. Isotypes of light strains of the three McAbs all belong to kappa. They were used in antigen-coated plate (ACP)-ELISA for CymMV detection, and ACP-ELISA could successfully detect 0.487 ng of purified CymMV or virus in plant sap diluted 1:10240. The presence of CymMV in field Orchids tissues was investigated with ACP-ELISA.
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