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  • Title: Development of artificial restriction DNA cutter composed of Ce(Iv)/EDTA and PNA.
    Author: Yamamoto Y, Uehara A, Miura K, Watanabe A, Aburatani H, Komiyama M.
    Journal: Nucleosides Nucleotides Nucleic Acids; 2007; 26(10-12):1265-8. PubMed ID: 18066765.
    Abstract:
    An artificial restriction enzyme, which we developed recently by combining Ce(IV)/EDTA and peptide nucleic acids, was used for PCR-free construction of a fusion protein. The fusion protein was successfully expressed in mammalian cells. This artificial DNA cutter can be also applied to site-selective scission of huge DNAs. Promising features of this novel tool were concretely evidenced.
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