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Title: Hydration regulates the thermodynamic stability of DNA structures under molecular crowding conditions. Author: Miyoshi D, Karimata H, Sugimoto N. Journal: Nucleosides Nucleotides Nucleic Acids; 2007; 26(6-7):589-95. PubMed ID: 18066861. Abstract: Although water is an integral part of DNA structures, the effects of water molecules on various DNA structures which are formed by not only Watson-Crick but also Hoogsteen base pairs are still unclear. Here, we studied quantitatively the effects of molecular crowding on the thermodynamics of a parallel G-quadruplex formation of [d(TG 4)2]4 with Hoogsteen base pairs. It was demonstrated that molecular crowding conditions stabilized the parallel G-quadruplex. Moreover, the plot of stability of the parallel G-quadruplex structure versus water activity suggested that water molecules were released through the G-quadruplex formation. The stabilization of the DNA structures consisting of Hoogsteen base pairs under cell-like conditions may lead to a structural polymorphism of various DNA sequences regulated by water molecules.[Abstract] [Full Text] [Related] [New Search]