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  • Title: Microarray based Raman spectroscopic detection with gold nanoparticle probes.
    Author: Li T, Guo L, Wang Z.
    Journal: Biosens Bioelectron; 2008 Feb 28; 23(7):1125-30. PubMed ID: 18068972.
    Abstract:
    A microarray approach based on surface-enhanced Raman spectroscopic (SERS) was developed for detection of spotted peptide, peptide-protein or protein-antibody interaction. The procedure involves the attachment of peptide-capped gold nanoparticles followed by silver deposition for signal enhancement. The attachment of the gold nanoparticles is achieved by standard avidin-biotin chemistry. The well-known biomolecular recognition pairs, IgG/protein A and biotin/avidin, were used to demonstrate proof-of-concept of the SERS assay. Detection limits of 10 and 100 fg per microarray spot were obtained respectively for the peptide and protein arrays. For the protein in solution, a limit of 0.1 microg/mL is reported. Furthermore, enzyme activity of the kinase (PKA) is also detected with high specificity for an established peptide substrate (kemptide) on the microarray spots.
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