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  • Title: [The expression of estrogen receptors in the human periodontal ligament fibroblasts in vitro].
    Author: Cao M, Ding Y, Shu L, Li J, Zhang W, Guo T.
    Journal: Zhonghua Kou Qiang Yi Xue Za Zhi; 2007 Sep; 42(9):533-6. PubMed ID: 18070427.
    Abstract:
    OBJECTIVE: To explore the expression of estrogen receptors in the human periodontal ligament fibroblasts in vitro. METHODS: Human periodontal ligament fibroblasts were cultured in vitro. The estrogen receptors (ER)-alpha, ER-beta were detected with immunocytochemistry staining; The mRNA of ER-alpha, ER-beta were measured by reverse transcription polymerase chain reaction; The expression of ER-alpha, ER-beta protein were detected by Western blot. RESULTS: The mRNA and protein expression of two subtype of ER were observed on human periodontal ligament fibroblasts (HPLF). The intensity of the ER-beta bands were stronger than those of ER-alpha, indicating that ER-beta expression in HPLF was higher. CONCLUSIONS: ER may play an important role in the function of human periodontal ligament fibroblasts.
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