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  • Title: High-cell-density cultivation for co-production of ergosterol and reduced glutathione by Saccharomyces cerevisiae.
    Author: Shang F, Wang Z, Tan T.
    Journal: Appl Microbiol Biotechnol; 2008 Jan; 77(6):1233-40. PubMed ID: 18071647.
    Abstract:
    Two different high-cell-density cultivation processes based on the mutant Saccharomyces cerevisiae GE-2 for simultaneous production of glutathione and ergosterol were investigated. Compared with keeping the ethanol volumetric concentration at a constant low level, feedback control of glucose feeding rate (F) by keeping the descending rate of ethanol volumetric concentration (DeltaE/Deltat) between -0.1% and 0.15% per hour was much more efficient to achieve a high glutathione and ergosterol productivity. This bioprocess overcomes some disadvantages of traditional S. cerevisiae-based cultivation process, especially shortening cultivation period and making the cultivation process steady-going. A classical on or off controller was used to manipulate F to maintain DeltaE/Deltat at its set point. The dry cell weight, glutathione yield and ergosterol yield reached 110.0 +/- 2.6 g/l, 2,280 +/- 76 mg/l, and 1,510 +/- 28 mg/l in 32 h, respectively.
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