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  • Title: [Study on HPLC fingerprint of water-soluble constituents of Carthamus tinctorius].
    Author: Tang XL, Liu J, Li GQ, Zhong HM, Qiu PJ, Zhang J.
    Journal: Zhong Yao Cai; 2007 Aug; 30(8):923-9. PubMed ID: 18074836.
    Abstract:
    OBJECTIVE: To establish the HPLC-fingerprint of the water-soluble constituents of Carthamus tinctorius. METHODS: 18 samples of Carthamus tinctorius from different producing areas were determined by Agilent 1100 DAD-HPLC under the chromatographic conditions: column by SinoChrom ODS-BP (250 mm x 4.6 mm, 5 microm), methanol-0.7% H3PO4 water with gradient elution, column temperature 30 degrees C, flow rate by 1 ml/min, wavelength 280 nm, and inject volume 20 microl. RESULTS: The HPLC-fingerprint of the water-soluble constituents of Cartharnus tinctorius was established on the basis of 10 bitch of drugs from Xinjiang according to SPSS analysis. CONCLUSION: A reliable method is provided for the quality identification of Carthamus tinctorius.
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