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  • Title: [Studies on the HPLC fingerprint spectrum of flavonoids in Gynostemma pentaphyllum].
    Author: Lu JQ, Chen L, Xiao B, Wan W, Yu F, Tian YP.
    Journal: Zhong Yao Cai; 2007 Aug; 30(8):929-32. PubMed ID: 18074837.
    Abstract:
    OBJECTIVE: To establish the HPLC fingerprint spectrum of flavonoids from Gynostemma pentaphyllum. METHODS: The HPLC conditions with TURNER Kromasil C18 column (250 x 4. 6 mm, 5 microm) was used, the mobile phase consisted of 0.4% phosphoric acid-methanol-acetonitrile with gradient elution at flow rate of 0.5 ml/min and the detection wavelength was set at 360nm. RESULTS: Ten common characteristic peaks were taken as fingerprint peaks the presision and the retest are in accordance with the requirement. CONCLUSION: The method is stable, reliable, precision and provides a scientific basis for the quality standard of Gynostemma pentaphyllum.
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