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  • Title: Novel reversed-phase high-performance liquid chromatography stationary phase with oligo(ethylene glycol) "click" to silica.
    Author: Guo Z, Liu Y, Xu J, Xu Q, Xue X, Zhang F, Ke Y, Liang X, Lei A.
    Journal: J Chromatogr A; 2008 May 16; 1191(1-2):78-82. PubMed ID: 18082752.
    Abstract:
    Oligo(ethylene glycol) (OEG) covalently bonded silica was prepared by using click chemistry and employed as a stationary phase for reversed-phase high-performance liquid chromatography. The column efficiency and effect of organic modifier content on retention were investigated. The separation selectivity was also studied with phenyl compounds and an actual sample of natural products. The results indicated that the stationary phase possessed good separation efficiency and separation selectivity in RP-HPLC mode. Moreover, the stationary phase showed good complementary separation selectivity to the C18 stationary phase. The OEG stationary phase had "clustering" function for "homologous component" in the separation of natural products.
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