These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: X and Y chromosome inheritance and mixtures of rDNA intergenic spacer regions in Anopheles gambiae.
    Author: Wilkins EE, Howell PI, Benedict MQ.
    Journal: Insect Mol Biol; 2007 Dec; 16(6):735-41. PubMed ID: 18093002.
    Abstract:
    We observed Anopheles gambiae sensu stricto stocks that contained both Mopti (M) and Savanna (S) rDNA intergenic spacers (IGS). ASEMBO1 male IGS sequences consistently had a mixture. A diagnostic M and S Hha I restriction enzyme site in these fragments was concordant with two SNPs associated with M and S. Standard M and S stocks demonstrated X-chromosome-only inheritance of the rDNA form, but the ASEMBO1 males showed X and Y chromosome linkage of mixed rDNA. The metaphase Y chromosomes of ASEMBO1 contained a significantly larger amount of DNA relative to the X than a standard S stock. Analysis of wild A. gambiae males from the putative location of origin of the ASEMBO1 stock did not detect the same pattern of polymorphism observed in the laboratory stock but did detect heterogeneous arrays including some missing a diagnostic Hha I restriction site. These results demonstrate that M and S IGS types can occur within the rDNA arrays of a chromatid in laboratory A. gambiae stocks, and some A. gambiae s.s. have rDNA on the Y chromosome.
    [Abstract] [Full Text] [Related] [New Search]