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  • Title: [The influence of angiotensin II receptor blockade on the expressions of Col I and TIMP-1 mRNA in cardiac fibroblast of adult rat].
    Author: Jiang XY, Gao GD.
    Journal: Sichuan Da Xue Xue Bao Yi Xue Ban; 2007 Nov; 38(6):954-7. PubMed ID: 18095594.
    Abstract:
    OBJECTIVE: To investigate the role of angiotensin II receptor type 2 (AT2) on collagen metabolism in cardiac fibroblast, and the difference between AT2 and angiotensin II receptors type 1 (AT1). METHODS: Adult rat cardiac fibroblasts were isolated and cultured. The cells were divided into 4 groups: Angiotensin II (Ang II), Ang II + Losartan, Ang II + PD123319, Ang II + Losartan + PD123319. Semiquantitative reverse transcription PCR was used to determine the mRNA levels of collagen I (Col I ) and tissue inhibitor of metalloproteinase 1 (TIMP-1). RESULTS: Losartan-treatment making AT1 blockade decreased Col I mRNA to 71.8% in cardiac fibroblasts, AT2 blockade from PD123319-treatment decreased Col I mRNA to 81.5%, co-treatment of both AT1 and AT2 blockade decreased Col I mRNA to 50.9% ; the AT1 blockade with Losartan-treatment decreased TIMP-1 mRNA to 88.1% in cardiac fibroblasts, AT2 blockade by PD123319-treatment decreased TIMP-1 mRNA to 75.4%, Both AT1 and AT2 blockade by co-treatment decreased TIMP-1 mRNA to 44.1%. CONCLUSION: AT2 receptor is involved in collagen metabolism in cardiac fibroblast by regulating the levels of Col I and TIMP-1 mRNA.
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