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  • Title: Sperm nuclear DNA fragmentation and mitochondrial activity in men with varicocele.
    Author: Blumer CG, Fariello RM, Restelli AE, Spaine DM, Bertolla RP, Cedenho AP.
    Journal: Fertil Steril; 2008 Nov; 90(5):1716-22. PubMed ID: 18155699.
    Abstract:
    OBJECTIVE: To verify the impact of varicocele on semen quality and sperm function (DNA integrity and mitochondrial activity). DESIGN: Prospective study. SETTING: Patients in an academic research environment. PATIENT(S): Seventeen patients with a clinical diagnosed varicocele of grade II or III and 20 men without a varicocele. MAIN OUTCOME MEASURE(S): Rate of sperm DNA fragmentation as assessed by the Comet assay and categorized as classes I (no DNA fragmentation), II (little DNA fragmentation), III (meaningful DNA fragmentation), and IV (high DNA fragmentation). Rate of mitochondrial activity as assessed by the diaminobenzidine (DAB) assay and categorized as grades I (all mitochondria active), II (most mitochondria active), III (most mitochondria inactive), and IV (all mitochondria inactive). RESULT(S): No statistically significant differences were found between the study and control groups with respect to age, ejaculatory abstinence, and round cell count. Men with varicocele had significantly higher ejaculate volume, concentration of immotile sperm, and neutrophil count and lower mean percentage of sperm concentration, progressive motility, and morphology than men in the control group. The study group presented a lower percentage of sperm with little DNA fragmentation (class II) and a higher percentage of sperm with DNA fragmentation (class IV). In addition, the study group presented a greater percentage of sperm with inactive mitochondria (class III). CONCLUSION(S): Compared with men without varicocele, men with varicocele had a higher percentage of cells with DNA fragmentation and sperm with inactive mitochondria. Indeed, varicocele causes a decrease in motility, concentration, and morphology and an increase in volume and concentration of immotile sperm and neutrophils. The sperm functional evaluation (DNA fragmentation and mitochondrial activity) could be important factors in deciding treatment options for men with varicocele.
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