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  • Title: Determination of dehydroalanine residues in proteins and peptides: an improved method.
    Author: Bartone NA, Bentley JD, Maclaren JA.
    Journal: J Protein Chem; 1991 Dec; 10(6):603-7. PubMed ID: 1815586.
    Abstract:
    Dehydroalanine residues in peptides and proteins react with 4-pyridoethanethiol in alkaline solution to form 4-pyridoethyl cysteine residues, which after acid hydrolysis, give the corresponding amino acid. An experimental protocol has been developed, and with simple dehydroalanine derivatives, conversions of 96-99% are achieved. Tests with peptides and proteins show that serine and cysteine/cystine residues do not interfere with this analytical procedure. A sample of wool keratin contained 57 mumol dehydroalanine/g.
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