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  • Title: Mycoplasmas regulate the expression of heat-shock protein genes through CIRCE-HrcA interactions.
    Author: Chang LJ, Chen WH, Minion FC, Shiuan D.
    Journal: Biochem Biophys Res Commun; 2008 Feb 29; 367(1):213-8. PubMed ID: 18164681.
    Abstract:
    Mycoplasmas in general are rarely exposed to severe environmental changes except during its colonization and infection processes. Genomic analysis indicates that Mycoplasma hyopneumoniae possesses the genes of a single sigma factor and the HrcA repressor of negative regulation of the heat-shock response. A perfect inverted repeat sequence (5'-CTGGCACTT-N(9)-AAGTGCCAA-3') upstream of the DnaK gene has also been identified. In the present study, we demonstrate the functionality of HrcA-CIRCE interactions using the gel electrophoretic mobility shift assay. The presence of the unique sigma factor, HrcA repressor, and the CIRCE-like sequences reveals that mycoplasmal species may all use the negative regulatory mechanism in the heat-shock response. It is conceivable that mycoplasmas may have evolved a single HrcA repressor-based mechanism which might be the most simple and economical way of controlling HSP gene expression.
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