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  • Title: Sensitivity and specificity of different methods for the isolation of Salmonella from pigs.
    Author: Bager F, Petersen J.
    Journal: Acta Vet Scand; 1991; 32(4):473-81. PubMed ID: 1818506.
    Abstract:
    Three different selective enrichment media, Rappaport-Vassiliadis broth (RV), selenite broth (SB) and Müller-Kauffmann tetrathionate broth (MKTB), in combination with plating on modified brilliant green agar (BGA), were compared for the isolation of Salmonella from samples of pig feces. These conventional methods were also compared with a new ELISA kit in conjunction with RV and SB enrichment. Of the conventional methods, enrichment in RV had a higher sensitivity and selectivity than SB and MKTB. Recovery of S. typhimurium from MKTB was significantly poorer than recovery of other serotypes. The combination of RV enrichment and ELISA was as good as the conventional method involving RV enrichment, with a similar high sensitivity and specificity. Tre salmonella opformeringsmetoder, Müller-Kauffmann tetrathionat (MKTB), selenit bouillon (SB) og Rappaport-Vassiliadis bouillon (RV), in-kuberet ved henholdsvis 43°C, 37°C og 42°C, blev sammenlignet med hensyn til sensitivitet og selektivitet ved undersøgelse af gødiningsprøver fra svin. Efter selektiv opformering blev salmonella påvist, dels traditionelt ved udsæd på modificeret brilliantgrøn agar (BGA), og dels immunologisk ved hjælp af et nyt kommercielt ELISA kit. RV havde en statistisk signifikant højere sensitivitet og selektivitet end både SB og MKTB. Opformering i MKTB gav en statistisk signifikant lavere påvisning af S. typhimurium end af andre serotyper. Den anvendte ELISA teknik, kombineret med opformering i RV havde en lige så høj sensitivitet og specificitet som ved traditionel udsæd fra RV-mediet.
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