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  • Title: [Specific features of vector sequences insertion in the genome of transgenic plants].
    Author: Permiakova NV, Deĭneko EV, Shumnyĭ VK.
    Journal: Genetika; 2007 Nov; 43(11):1501-10. PubMed ID: 18186189.
    Abstract:
    Upon processing of T-DNA, both borders may be recognized incorrectly. A number of transgenic plants containing, apart from a fragment of vector DNA adjacent to the left border of T-DNA, a vector fragment inserted irrespective of it were identified. Sequence analysis confirmed that genes located beyond the boundaries of T-DNA are transferred and inserted in the genome of transgenic plants. Recombination of vector DNA was discovered among plants of the first generation after self-pollination of the original transformants.
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