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  • Title: Assay and purity control of metacycline by thin-layer chromatography combined with UV and fluorescence densitometry--a comparison with liquid chromatography.
    Author: Naidong W, Hua S, Verresen K, Roets E, Hoogmartens J.
    Journal: J Pharm Biomed Anal; 1991; 9(9):717-23. PubMed ID: 1821148.
    Abstract:
    A thin-layer chromatographic (TLC) method involving UV and fluorescence densitometry is described for the assay and purity control of metacycline. With a mobile phase dichloromethane-methanol-water (58:35:7, v/v/v) and a silica gel thin-layer, previously sprayed with 10% sodium edetate solution adjusted to pH 9.0, all the potential impurities of metacycline were well separated from the main component and from each other. Results obtained with UV densitometry (TLC-UV) and fluorescence densitometry (TLC-F) were compared with those obtained by a liquid chromatography (LC) method using a poly(styrene-divinylbenzene) stationary phase. The correlation coefficients (r) for TLC-UV and LC or TLC-F and LC were better than 0.9999. For TLC-UV the relative standard deviation (RSD) for the assay of the main component was less than 2%, for TLC-F less than 3.0% and for LC less than 1.0%.
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