These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: The role of beta-1,4-galactosyltransferase-I in the skin wound-healing process.
    Author: Shen A, Qian J, Liu L, Liu H, Chen J, Niu S, Yan M, Chen X, Shen C, Gu J, Cheng C.
    Journal: Am J Dermatopathol; 2008 Feb; 30(1):10-5. PubMed ID: 18212537.
    Abstract:
    Cell-surface carbohydrate chains are known to contribute to cell migration, interaction, and proliferation. beta-1,4-galactosyltransferase-I (beta-1,4-GalT-I), which is one of the best-studied glycosyltransferases, plays a key role in the synthesis of type 2 chains in N-glycans and the core 2 branch in O-glycans. Recently, it has been reported that skin wound healing is significantly delayed in beta-1,4-GalT-I mice. However, the expression of beta-1,4-GalT-I and its biological function in the skin wound-healing process remain to be elucidated. We used real-time polymerase chain reaction to demonstrate that the expression of beta-1,4-GalT-I mRNA reached plateau values at 12 hours after skin was injured and remained elevated until 11 days after the injury. Furthermore, lectin blotting showed that beta-1,4-galactosylated carbohydrate chains were also increased after skin injury. A double-staining method combining lectin-fluorescent staining with RCA-I and immunofluorescence was first used to determine the cellular localization of beta-1,4-galactosylated carbohydrate chains. Morphological analysis showed that the chains were primarily expressed in neutrophils and partially expressed in macrophages, endothelial cells, and collagen. Our results suggest that beta-1,4-GalT-I and beta-1,4-galactosylated carbohydrate chains participate in leukocyte recruitment, angiogenesis, and collagen deposition in the skin wound-healing process.
    [Abstract] [Full Text] [Related] [New Search]