These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Screening of Bacillus sp. strains producing thermostable alpha-amylase].
    Author: Kubrak OI, Lushchak VI.
    Journal: Mikrobiol Z; 2007; 69(5):26-34. PubMed ID: 18217351.
    Abstract:
    Fifteen thermostable alpha-amylases have been obtained as a result of screening of 76 Bacillus sp. strains, isolated from different natural substrates. They have shown 30-100% of their activities after 60 min of incubation at 100 degrees C. Seven thermostable a-amylases demonstrated 100% of their activities after 60 min of incubation at 100 degrees C. These enzymes showed maximal activities at pH 9.0 and exhibited 10-30% of activities at pH 11.0 and 12.0. These properties make them promising for future research and possible practical use. In this paper we have proposed a modified iodometric method for measurement and calculation of alpha-amylases activity. The influence of ammonium sulfate on protein concentration measurement by Bradford method was demonstrated and a necessity of dialysis after precipitation with ammonium sulfate was proposed.
    [Abstract] [Full Text] [Related] [New Search]