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  • Title: Matrix metalloproteinases.
    Author: Birkedal-Hansen H, Yamada S, Windsor J, Poulsen AH, Lyons G, Stetler-Stevenson W, Birkedal-Hansen B.
    Journal: Curr Protoc Cell Biol; 2003 Feb; Chapter 10():Unit 10.8. PubMed ID: 18228416.
    Abstract:
    Matrix metalloproteinases are a class of enzymes that play an important role in the remodeling of the extracellular matrix in development and cancer metastasis. This unit describes a set of methods-cell-mediated dissolution of type I collagen fibrils, direct and reverse zymography, enzyme capture based on a-2 macroglubulin and TIMP-1 and -2, and demonstration of crytic thiol groups in metalloproteinase precursors-that are used to characterize the functions of matrix metalloproteinases and their inhibitors.
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