These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: In vitro analysis of chloroplast protein import.
    Author: Smith MD, Schnell DJ, Fitzpatrick L, Keegstra K.
    Journal: Curr Protoc Cell Biol; 2003 Feb; Chapter 11():Unit11.16. PubMed ID: 18228418.
    Abstract:
    This unit describes protocols for isolating chloroplasts from pea (Pisum sativum) and Arabidopsis thaliana for the study of nuclear-encoded plastid precursor proteins. Chloroplasts from both preparations are competent for the in vitro import of recombinant preproteins synthesized using in vitro translation systems derived from reticulocyte or wheat germ lysates. These assays can be used to test whether a particular protein is targeted to chloroplasts, for analyzing the suborganellar location of newly imported preproteins, or to study the mechanism of import itself.
    [Abstract] [Full Text] [Related] [New Search]