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  • Title: A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis.
    Author: Kenefic LJ, Beaudry J, Trim C, Huynh L, Zanecki S, Matthews M, Schupp J, Van Ert M, Keim P.
    Journal: J Microbiol Methods; 2008 Jun; 73(3):269-72. PubMed ID: 18237793.
    Abstract:
    The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.
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