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  • Title: Simultaneous separation and determination of coenzyme Q(10) and its process related impurities by NARP-HPLC and atmospheric pressure chemical ionization-mass spectrometry (APCI-MS).
    Author: Nageswara Rao R, Kumar Talluri MV, Shinde DD.
    Journal: J Pharm Biomed Anal; 2008 Jun 09; 47(2):230-7. PubMed ID: 18243624.
    Abstract:
    A non-aqueous reversed phase high performance liquid chromatographic (NARP-HPLC) method for determination of coenzyme Q(10) in pharmaceutical preparations has been developed using Kromosil C(8) column with acetonitrile and isopropyl alcohol (84:16, v/v) as a mobile phase. Photodiode array (PDA) detector set at 210 nm was used for monitoring of the eluents. The method is simple, rapid, selective and capable of separating all process impurities at trace level with detection limits <0.1 microg/ml. It has been validated with respect to accuracy, precision, linearity, and limits of detection and quantification. The linearity range was 50-300 microg/ml. The percentage recoveries ranged from 95.10 to 101.02. The method was found to be suitable not only for monitoring the reactions during the process development but also quality assurance of coenzyme Q(10). For identification of related substances atmospheric pressure chemical ionisation-mass spectrometry (APCI-MS) was used.
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