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  • Title: Correlation between a novel calpastatin biosensor and traditional calpastatin assay techniques.
    Author: Bratcher CL, Grant SA, Stringer RC, Lorenzen CL.
    Journal: Biosens Bioelectron; 2008 May 15; 23(10):1429-34. PubMed ID: 18243684.
    Abstract:
    An optical fiber biosensor to detect calpastatin has been investigated as a preliminary step in developing tenderness detection instrumentation. Longissimus dorsi samples were taken from beef carcasses (n=21) at 0, 24, 36 and 48h postmortem. Muscle homogenates were assayed for calpastatin activity using traditional methods and an optical fiber biosensor. Warner-Bratzler shear force was also performed on a steak from each carcass at 14d postmortem. Results demonstrated that the measurements with highest correlation between traditional calpastatin assays and optical biosensor readings were taken at 48h postmortem (r=0.597, P< or =0.01), suggesting that this is the best time for use of this biosensor in an on-line grading system. This research further advances the development of a calpastatin biosensor and would be useful in laboratory determination of the presence of biologically active calpastatin concentrations.
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