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  • Title: Eukaryotic expression and antimicrobial spectrum determination of the peptide tachyplesin II.
    Author: Xu F, Meng K, Wang YR, Luo HY, Yang PL, Wu NF, Fan YL, Yao B.
    Journal: Protein Expr Purif; 2008 Apr; 58(2):175-83. PubMed ID: 18249136.
    Abstract:
    Ta0-a, the gene encoding the mature antimicrobial peptide tachyplesin II, was engineered to optimize the coding sequence according to codon usage bias in yeast. Ta0-a was efficiently expressed in the methylotrophic yeast Pichia pastoris strain SMD1168. The recombinant peptide Ta0 reached 150mg/L after methanol induction for 6 d. Ta0 was rapidly purified to homogeneity by a single step of size-exclusion chromatography. The minimal lethal concentrations of Ta0 to the Escherichia coli strain K12 was 30 microg/mL. Ta0 exhibited a wide range of antimicrobial activity: the growth of 26 bacterial and fungal strains, including some typical food/feed spoilage microorganisms, was all substantially inhibited. This result indicates the potential practical application of the recombinant peptide in various industrial products.
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