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  • Title: Hapten synthesis and monoclonal antibody-based immunoassay development for the detection of the fungicide kresoxim-methyl.
    Author: Mercader JV, Suárez-Pantaleón C, Agulló C, Abad-Somovilla A, Abad-Fuentes A.
    Journal: J Agric Food Chem; 2008 Mar 12; 56(5):1545-52. PubMed ID: 18251498.
    Abstract:
    Strobilurin fungicides have been increasingly used for fungus pest control since they were introduced in 1996. For pesticide residue detection, immunoassays constitute nowadays a valuable approach. This paper describes the synthesis of functionalized haptens of kresoxim-methyl, the production of monoclonal antibodies, and the development of enzyme-linked immunosorbent assays. On the one hand, a two-step conjugate-coated immunoassay was optimized using extended or short incubation times, with limits of detection of 0.4 ng/mL for the extended assay and 0.3 ng/mL for the rapid assay. On the other hand, an immunoassay was optimized following a procedure consisting of just one incubation step. This one-step assay had a limit of detection of 0.4 ng/mL. All of these assays showed a similar performance, with sensitivities well below common maximum residue limits for this pesticide (50 microg/kg) and lower than the detection limits of the usual chromatographic detection methods.
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