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  • Title: Technical note: improved ancient DNA purification for PCR using ion-exchange columns.
    Author: Kim K, Kim KY, Jeon E, Togloom A, Cho YO, Lee MS, Lkhagvasuren G, Choi JH, Tumen D, Ja Park A, Kim KC, Park KW, Kim JH, Noh M, Yoo KJ, Lee KH.
    Journal: Am J Phys Anthropol; 2008 May; 136(1):114-21. PubMed ID: 18257014.
    Abstract:
    A novel method of ancient DNA (aDNA) purification was developed using ion-exchange columns to improve PCR-amplifiable DNA extraction from ancient bone samples. Thirteen PCR-resistant ancient bone samples aged 500-3,300 years were tested to extract aDNA using a recently reported, silica-based aDNA extraction method and an ion-exchange column method for the further purification. The PCR success rates of the aDNA extracts were evaluated for the amplification ability of the fragments of mitochondrial DNA, a high-copy DNA, and amelogenin, a low-copy DNA. The results demonstrate that the further purification of silica-based aDNA extracts using ion-exchange columns considerably improved PCR amplification. We suggest that the ion-exchange column-based method will be useful for the improvement of PCR-amplifiable aDNA extraction, particularly from the poorly preserved, PCR-resistant, ancient samples.
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