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  • Title: [Cloning and sequence analysis of SOCS-2 gene in pig].
    Author: Du BW, Yang GS, Sun C.
    Journal: Sheng Wu Gong Cheng Xue Bao; 2007 Nov; 23(6):1091-6. PubMed ID: 18257242.
    Abstract:
    Total RNA was isolated from kidney of BaMei pig, a local strain of Chinese pig, and then the cDNA sequence of SOCS-2 gene was cloned by RT-PCR (GenBank accepted number is EF121242). Then the cloned SOCS-2 gene was inserted into PMD19-T vector by T/A cloning, transformed into DH-5alpha, tested by PCR and sequenced. The data show that the homology of the cloned porcine SOCS-2, including 822 bp, is more than 93% and that of the deduced amino acid sequence is 89% when compared with human, rat and mice. And the molecular weight of SOCS-2 protein is about 22.25 kD and PI is 8.03. The cloning of SOCS-2 gene is useful for the further research on the molecular mechanism by which regulating growth and development of organism.
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