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  • Title: Isolation, characterization, and sequence of an Escherichia coli heat shock gene, htpX.
    Author: Kornitzer D, Teff D, Altuvia S, Oppenheim AB.
    Journal: J Bacteriol; 1991 May; 173(9):2944-53. PubMed ID: 1826904.
    Abstract:
    We isolated and characterized a new Escherichia coli gene, htpX. The htpX gene has been localized at min 40.3 on the chromosome. We determined its transcription and translation start site. htpX expresses a 32-kDa protein from a monocistronic transcript; expression of this protein is induced by temperature upshift. htpX is expressed from a sigma 32-dependent promoter and is thus part of the heat shock regulon. Cells carrying a htpX gene disruption grow well at all temperatures and under all conditions tested and have no apparent phenotype. However, cells which overexpress a truncated form of the protein display a higher rate of degradation of puromycyl peptides.
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