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  • Title: Nuclease tolerant FRET probe based on DNA-quantum dot conjugation.
    Author: Onoshima D, Kaji N, Tokeshi M, Baba Y.
    Journal: Anal Sci; 2008 Feb; 24(2):181-3. PubMed ID: 18270406.
    Abstract:
    We have developed a fluorescence resonance energy transfer (FRET) probe based on the conjugation of a quantum dot (QD) with dye (YOYO-3) intercalated DNA. The FRET-inducing electrostatic coupling of DNA and the QD made structural changes to the QD-DNA conjugates, which significantly prevented an enzymatic reaction between the DNA and a conventional restriction endonuclease (EcoRI).
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