These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Sensing single base incorporation with nanopore micromanipulation.
    Author: Croquette V.
    Journal: ACS Chem Biol; 2008 Feb 15; 3(2):92-4. PubMed ID: 18278849.
    Abstract:
    Using single-molecule techniques, scientists can routinely investigate the action of a single enzyme. The goal of such studies is usually to gain accurate information unachievable in ensemble assays, such as the maximum instantaneous rate of reaction, the existence of pauses or backward steps, etc. In the article discussed here, the authors have increased their experimental sensitivity so that they can detect a single enzymatic cycle. This improvement makes possible the use of a polymerase enzyme to sequence a single DNA molecule.
    [Abstract] [Full Text] [Related] [New Search]