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  • Title: Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study.
    Author: Scott R, Seli E, Miller K, Sakkas D, Scott K, Burns DH.
    Journal: Fertil Steril; 2008 Jul; 90(1):77-83. PubMed ID: 18281045.
    Abstract:
    OBJECTIVE: To determine if metabolomic profiling of embryonic development was associated with implantation rates in IVF. DESIGN: Prospective blinded. SETTING: University-affiliated assisted reproductive technology program. PATIENT(S): Unselected assisted reproductive technology population. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Raman-based biospectroscopic metabolomic profiling of spent culture media and delivery rates. RESULTS: Forty-one spent media samples from 19 patients with known reproductive potential (0 or 100% delivery rates of each embryo that implanted) were evaluated. Raman-based metabolomic profiling was used to calculate a viability index for each sample. On day 3, the spent media of embryos with proven reproductive potential (n = 33) demonstrated higher viability indices (0.875 +/- 0.12) than those that failed to implant (0.56 +/- 0.09). Similar findings were present in spent media from embryos transferred on day 5 (n = 8) (-0.40 +/- -0.21 vs. -0.81 +/- -0.08). Receiver operating characteristic curve analyses were used to select thresholds with the greatest ability to discriminate outcomes. Overall diagnostic accuracy for predicting delivery or a failed implantation was 80.5%. CONCLUSIONS: There is a clear relationship between the reproductive potential of human embryos and their modification of their culture media as detected by Raman biospectroscopy-based metabolomic profiling. This technology offers great potential for development as a tool to allow rapid noninvasive assessment of embryonic reproductive potential before transfer.
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