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  • Title: Amperometric immunosensor for diagnosis of BLV infection.
    Author: Kurtinaitiene B, Ambrozaite D, Laurinavicius V, Ramanaviciene A, Ramanavicius A.
    Journal: Biosens Bioelectron; 2008 May 15; 23(10):1547-54. PubMed ID: 18294837.
    Abstract:
    A new amperometric immunosensor for detection of antibodies against bovine leukemia protein (gp51) was designed. The detection of antibody-antigen complex formation was based on application of secondary antibodies labeled with horseradish peroxidase (HRP). Ferrocenecarboxylic acid (FCA) and N,N,N',N'-tetramethylbenzidine (TMB) were selected as suitable mediators for this immunosensor. Optimal conditions for amperometric detection were found. Sensitivity of created system was compared with the results of enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) reaction, and was sufficient for detection of usual anti-gp51 antibody concentration present in the blood serum of BLV-infected cattle.
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