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  • Title: [Enrichment of type A1-A4 spermatogonia by flow cytometry].
    Author: Bai Y, Ye ZW, Zeng FQ.
    Journal: Zhonghua Nan Ke Xue; 2008 Jan; 14(1):3-6. PubMed ID: 18297801.
    Abstract:
    OBJECTIVE: To explore a method to isolate and purify the subtype of type A spermatogonial stem cells (SSCs). METHODS: We isolated spermatogonia by discontinuous density gradient centrifugation, sorted c-kit-expressed cells with the fluorescence-activated cell sorter (FACS), observed their ultrastructure by electron microscope, and performed immunohistochemistry to determine the expression of c-kit in the testis. RESULTS: The c-kit positive cells constituted (18.65 +/- 1.69) % of the testis cells that were isolated by density gradient centrifugation, but made up only (3.16 +/- 0.84) % of those that were not (P < 0.01). The rates of recovery and viability of the c-kit positive cells sorted by FACS were (65.90 +/- 1.24)% and (85.60 +/- 1.14)%, respectively. CONCLUSION: With c-kit as the marker, FACS can effectively isolate and purify the subtype of SSCs after preliminarily purified by discontinuous density gradient centrifugation.
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