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  • Title: [Effects of two different cryoprotectants on the motility of post-thaw human sperm].
    Author: Hu JM, Xu CY, Li Y, Lu SM, Wang L, Chen ZJ.
    Journal: Zhonghua Nan Ke Xue; 2008 Jan; 14(1):23-5. PubMed ID: 18297806.
    Abstract:
    OBJECTIVE: To compare the effects of the cryoprotectant containing glucose and that containing sucrose on the motility of post-thaw human sperm. METHODS: The cryoprotectant containing glucose and that containing sucrose were applied to 50 semen samples and the motility of the post-thaw human sperm was compared before and after cryopreservation and between the study groups. RESULTS: The forward motility and total motility of the sperm were (58.4 +/- 5.7)% and (63.4 +/- 6.1)% before cryopreservation, (43.8 +/- 7.6)% and (48.4 +/- 7.6)% after thawing with the cryoprotectant containing glucose, and(42.6 +/- 8.9)% and (48.0 +/- 8.5)% after thawing with the cryoprotectant containing sucrose. Decreased sperm motility was observed after cryopreservation, with statistic significance (P < 0.01). There was no significant difference in the forward and total motility of the post-thaw sperm between the two cryoprotectants. CONCLUSION: Cryopreservation inflicts obvious damage on sperm. Sucrose is a feasible sperm cryoprotectant.
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