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  • Title: [Use of DNA polymorphism detected by M13 phage DNA in population studies].
    Author: Barysheva EV, Bukina AM, Petrova NV, Limborskaia SA, Ginter EK.
    Journal: Genetika; 1991 Mar; 27(3):399-403. PubMed ID: 1830281.
    Abstract:
    Hypervariable "minisatellite" regions detected in human genome by wild-type phage M13 DNA were found to have high polymorphism and somatic stability. Analysis of individual specific patterns of hybridization of 44 human DNAs from the Kirov province is presented. Molecular weight of fragments varied from 2 to 6 kb. Mean frequency of a fragment in the population under study is p = 0.294 +/- 0.158. The mean number of fragments per individual is 11.6 +/- 1.8. Comparison between the Kirov population and that of Krasnodar studied earlier was carried out. The mean genetic distance between Kirov and Krasnodar populations calculated according to Nei is 0.2082. The possibility of using in population-genetic studies of hypervariable DNA markers having fingerprint type of hybridization is discussed.
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