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  • Title: Arabidopsis mitochondrial proteomics.
    Author: Heazlewood JL, Millar AH.
    Journal: Methods Mol Biol; 2007; 372():559-71. PubMed ID: 18314751.
    Abstract:
    Significant efforts have sought to uncover the protein profile of Arabidopsis mitochondria to act as a model for the mitochondrial proteome from plants. A combination of techniques have been undertaken to achieve this goal. We outline a basic two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) separation of mitochondrial proteins, in-gel trypsination techniques, complex protein lysate digestions, and the identification of proteins by matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) mass spectrometry.
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