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  • Title: [Studies on the methods of extract and determination of total tannins and gallic acids of the root of Euphorbia hylonoma].
    Author: Guo ZJ, Sun QS, Long LH, Bu XQ.
    Journal: Zhong Yao Cai; 2007 Nov; 30(11):1398-401. PubMed ID: 18323208.
    Abstract:
    OBJECTIVE: To determine total tannins and gallic acid of the root of Euphorbia hyloomla. METHODS: With the gallic acid as reference, tannins and gallic acid of the root of Euphorbia hylonoma were determined by ultraviolet spectrophtometer and high performance liquid chromatography, respectively. RESULTS: The content of tannins of the root of Euphorbia hylonoma were determined by different extract methods, including extracted by water, 70% alcohol, acetone on supersound, the determination data were 4.375%, 7.240%, 3.958%, respectively. When used recirculation method by water, 70% alcohol, acetone, the determination data were the determination data are 3.773%, 2.503%, 1.59%, respectively. The content of gallic acid of the root was 0.047%. CONCLUSION: Content of total tannins by alcohol super sound is the highest comparing with other extract methods, this method can used in extract of total Tannins of the root of Euphorbia hylonoma.
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