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  • Title: The mitochondrial ATP synthase of Trypanosoma brucei: developmental regulation through the life cycle.
    Author: Williams N, Choi SY, Ruyechan WT, Frank PH.
    Journal: Arch Biochem Biophys; 1991 Aug 01; 288(2):509-15. PubMed ID: 1832842.
    Abstract:
    The mitochondrial H(+)-ATPase of the parasitic protozoan Trypanosoma brucei is shown to be developmentally regulated through the T. brucei life cycle as has been shown for components of the mitochondrial electron transport chain. We have substantiated our results by assaying not only for oligomycin-sensitive ATPase activity but also by determining the level of ATP synthetic activity. These results show that the level of ATPase present in the procyclic form of T. brucei is increased by at least threefold from that of the early bloodstream form while the ATPase activity in the late bloodstream form is only about twofold higher than the early form. ATP synthesis activity shows these same results. We have determined the level of ATP synthase protein present in the life cycle stages by Western analysis employing the antibodies that we have raised against both the water soluble F1 and the membrane-associated F0 moieties which we have purified from T. brucei. The Western blots of the procyclic form show strong reactivity with both the F0 and F1 antibodies. The other two life cycle stages, the early and the late bloodstream forms, show considerably less reactivity, paralleling the activity results. Electron micrographs of the sonicated mitochondrial fraction show inverted vesicles which are studded with knobby H(+)-ATPase in the procyclic form. The early bloodstream vesicles show very few of these characteristic structures, while the late bloodstream form shows a range of vesicles from nearly nude to partially studded.
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