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  • Title: Comparison of two types of endothelial cells in long term culture.
    Author: Sedlak BJ, Booyse FM, Bell S, Rafelson ME.
    Journal: Thromb Haemost; 1976 Feb 29; 35(1):167-77. PubMed ID: 183305.
    Abstract:
    Bovine aortic endothelial cells have been serially subcultured and maintained for ten months (24 passages) without any apparent loss of endothelial-like properties. There appear to be two endothelial cell types; one type is mononucleated, 40-60 mu in diameter, and the other (giant) cell type, multinucleate and 300-600 mu in diameter. Both cell types were identified as endothelial cells by established criteria; 1. ultrastructurally, by the presence of Weibel-Palade bodies, microfilaments and pinocytotic vesicles and 2. immunologically, by the presence of factor VIII antigen and thrombosthenin.
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