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  • Title: An optical rotatory detector for high-performance liquid chromatography using polarization modulation.
    Author: Yamamoto A, Kawai M, Sakamoto M, Kodama S, Hayakawa K.
    Journal: Anal Sci; 2008 Mar; 24(3):361-4. PubMed ID: 18332543.
    Abstract:
    A sensitive and variable-wavelength optical rotatory (OR) detector for high-performance liquid chromatography is presented. This design is entirely different from that of conventional OR detectors consisting of a crossed polarizer pair. By placing a polarizing prism and a retardation plate into a commercial circular dichroism (CD) detector, the OR signal was obtained. The Mueller matrix approach was used to prove the principle of the OR signal appearance. Sugars and 4-androstene-3,17-dione were chosen as test compounds. The limit of detection was below 0.5 microg of injected sucrose at 260 nm, which was superior to that obtained with a conventional OR detector. For 4-androstene-3,17-dione, which is CD active, and shows a large anomalous OR dispersion curve, our detector gave a large OR signal with approximately half the intensity of the CD signal at 340 nm.
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